2017-07-24 14:10:552025-05-24 03:13:48
locus
BSU28620
BSU_28620
function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules, processing of R-loops, maturation of Okazaki fragments
product
Mg2+-dependent RNase HIII
Mn2+-dependent RNase HIII
outlinks
bsu
BSU28620
BSU_28620
Gene
Phenotypes of a mutant
a ''[[gene|rnhB]] [[gene|rnhC]]'' double mutant grows poorly [Pubmed|23882084]
cold-sensisitive due to a defect in Okazaki fragment maturation [pubmed|30670546]
a [[gene|rnhC]] [[gene|polA]] double mutant is not viable at 25°C [pubmed|30670546]
a [[gene|rnhC]] [[gene|exoR]] double mutant is not viable at 25°C [pubmed|30670546]
sensitive to hydroxyurea [pubmed|29084857]
a ''[[gene|rnhB]] [[gene|rnhC]]'' double mutant grows poorly [Pubmed|23882084]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of RNA to 5'-phosphomonoester (according to Swiss-Prot)
maturation of Okazaki fragments (removal of RNA) (together with [[protein|PolA|DNA polymerase I]]) [pubmed|30670546]
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
The protein
Protein family
RnhC subfamily (according to Swiss-Prot)
[SW|RNase] HII family (with [[protein|RnhB]], according to UniProt)
The protein
[SW|Cofactors]
Mg2+
Mn2+ [pubmed|29084857]
The protein
Structure
[PDB|2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity)
[PDB|2D0A] (Geobacillus stearothermophilus, 47% identity)
[PDB|2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity) [pubmed|16343535]
[PDB|2D0A] (Geobacillus stearothermophilus, 47% identity) [pubmed|16343535]
Biological materials
Mutant
MGNA-B002 (ysgB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1001 NBRP B. subtilis, Japan]
BP430 (''ΔrnhC::cat'') available in [SW|Fabian Commichau]'s lab
MGNA-B002 (ysgB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1001 NBRP B. subtilis, Japan]
BP430 (''ΔrnhC::cat'') available in [SW|Fabian Commichau]'s lab
BKE28620 (Δ[[gene|rnhC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTAATAATCTCCTTTTT, downstream forward: _UP4_TAGAAAAAAGCTTGCAGATT
BKK28620 (Δ[[gene|rnhC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTAATAATCTCCTTTTT, downstream forward: _UP4_TAGAAAAAAGCTTGCAGATT
Biological materials
Expression vector
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in [SW|Fabian Commichau]'s lab
References
Reviews
References
Original publications
ec
3.1.26.4
The protein
Paralogous protein(s)
[[this]]
The protein
additional information
[[protein|RnhC]] may interact with [SW|RNA polymerase] [pubmed|21710567]
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in [SW|Fabian Commichau]'s lab